Expression Analysis of BIRC3 as One Target Gene of Transcription Factor NF-κB for Esophageal Cancer

Esophageal cancer (ESCA) is one of the highest lethal malignancy tumors worldwide. Baculoviral IAP repeat-containing protein 3 (BIRC3) main inhibitor apoptosis in many malignancies. The aim this study was to clarify how BIRC3 acts ESCA cells. Through TNMplot and GEPIA2 analysis, found abundantly expressed quantitative RT-PCR assay confirmed pronouncedly induced all used cell lines. In addition, proinflammatory cytokines TNFα IL-1β were shown have promotion effects on expression These promotive blocked when function NF-κB inhibited by bay 11-7082, which indicates gene regulated via transcription pathway ESCA. Moreover, bioinformatics analysis showed that had binding cis-elements. Chromatin immunoprecipitation then performed it directly interacts with cis-elements gene. conclusion, our data proved high level maintained survival up-regulated cytokine through signaling pathway, may be helpful for esophageal prevention therapy.